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IGOR PROCEDURE FOR ANALYSIS

Data Analysis

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Igor Procedure

MATLAB ROUTINE FOR BEAD TRACKING

Particle Tracking

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MatLab Routine

SOFTWARE FOR SINGLE MOLECULE MT-DATA

Step size detector

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MT Analysis v15

SOFTWARE DEMO FOR BROWNIAN MOTION

Energy Landscapes

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SCRIPT FOR FC SMD

Molecular Dynamics

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SCRIPT FOR SMD

Molecular Dynamics

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MULTIMEDIA

STEERED MOLECULAR DYNAMICS

Aug 16, 2020

Streptococcus pyogenes is formidable colonizer of human epithelium. One of the reason is the presence of covalent bonds in the pili proteins that prevents the mechanical unfolding during the adhesion. This bond is the isopeptide bond, which is made between the side chain of a Lysine and Asparigine residue.

GRABBING SINGLE MOLECULES

Jul 16, 2019

One of the Very first single molecule recording on SOPHIA after of reassembly at the CGB Univ Mayor. Protein: I10 titin domain.

TRACKING A SINGLE BEAD

July 12, 2019

How molecules diffuse inside the cell? Using a small speaker, a Petri dish and two cell phones–for tracking and video recording–, we calculated the diffusion coefficient for the red particle (bead). We use a short MatLab routine to track the red bead along the video. Video and MatLab script developed with the help of Romina Muñoz at SMAT-C USACH. Check files above.

HOW TO ASSEMBLY YOUR FOLDSCOPE

Aug 16, 2020

Video con las instrucciones paso a paso como entablar el microscopio origami FoldScope. Video preparado por Tomas.

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FILES

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THESIS ON TITIN ELASTICITY

Muscles are made of three main filaments: actin, myosin, and titin. Whereas the first two generate active contractile work, titin determinates the elasticity of the sarcomere. We are looking for highly motived student to join the lab on the exciting field of titin elasticity. Download the ad for more the details.

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THESIS ON BACTERIAL ADHESION

Did you know that bacterias withstand hundreds of picoNewtons of force before detaching? We are looking student to join a new project that aims to understand the mechanisms involved on the bacterial adhesion. Download the ad with the details.

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PROTOCOL FOR HALO-SURFACES

The file contains step-by-step protocol for the preparation of Halo-ligand glass surfaces. These functionalized coversildes could be use either for AFM or MT experiments. Download the file for more details.

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AFM DATA ANALYSIS

Igor Procedure for the analysis of AFM-FX and AFM-FC experiments. The file is based on the software developed by Fernandez lab (Columbia Univ). The file was written on Igor v6.2, but also can be run on newer version (v7 and v8).

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AFM ANALYSIS MULTIPLE LC

Igor Procedure for the analysis of AFM-FX including with a new module for the fitting of multiple Lc of FX. The file is based on the software developed by Fernandez lab (Columbia Univ). The file was written on Igor v6.2, but also can be run on newer version (v7 and v8).

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MT STEP COUNTER

Software based on Igor (v7) for importing and fitting data collected on MT experiments. The panel allow to navigate through the trace recording the step size and the time of the event. Also allows to visualize consolidated step size distributions from the (un)folding events present on the recordings.

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FILE NAME

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FILE NAME

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